![]() Spring scissors (15004-08 Fine Science Tools) Multidish four-well plate (176740, Thermo scientific) Petri dish 90 mm (101RT/C, Thermo Scientific) Secondary antibodies were goat-anti-rabbit 647, goat-anti-mouse 568 and goat-anti-chicken 488 diluted 1:1000 in blocking buffer.ĭissecting/stereo microscope (EZ4 HD, Leica) Primary antibodies used were glial fibrillary acidic protein (GFAP), neuronal nuclei (NeuN) and microtubule-associated protein 2 (Map2) ( Table 1) at 1:1000 for GFAP and NeuN and 1:10000 for Map2 dilution in the blocking buffer. The final solution was filtered with a Whatman filter paper (pore size 45 mm). Poly- l-lysine working solution was made as 1:10 dilution of stock Poly- l-lysine in Milli-Q H 2O. Make upto 1 l with Milli-Q H 2O and autoclaved at 121☌ for 20 min.ĭulbecco’s modified Eagle’s medium (DMEM) ++ġ0 μg DNase I in 10 ml Preparation medium as above. ![]() Phosphate-buffered saline (PBS) buffer pH = 7.4
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